TriAnnot: A Versatile and High Performance Pipeline for the Automated Annotation of Plant Genomes.

TitleTriAnnot: A Versatile and High Performance Pipeline for the Automated Annotation of Plant Genomes.
Publication TypeJournal Article
Year of Publication2012
AuthorsLeroy, Philippe, Guilhot Nicolas, Sakai Hiroaki, Bernard Aurélien, Choulet Frédéric, Theil Sébastien, Reboux Sébastien, Amano Naoki, Flutre Timothée, Pelegrin Céline, Ohyanagi Hajime, Seidel Michael, Giacomoni Franck, Reichstadt Mathieu, Alaux Michael, Gicquello Emmanuelle, Legeai Fabrice, Cerutti Lorenzo, Numa Hisataka, Tanaka Tsuyoshi, Mayer Klaus, Itoh Takeshi, Quesneville Hadi, and Feuillet Catherine
JournalFrontiers in plant science
Date Published2012

In support of the international effort to obtain a reference sequence of the bread wheat genome and to provide plant communities dealing with large and complex genomes with a versatile, easy-to-use online automated tool for annotation, we have developed the TriAnnot pipeline. Its modular architecture allows for the annotation and masking of transposable elements, the structural, and functional annotation of protein-coding genes with an evidence-based quality indexing, and the identification of conserved non-coding sequences and molecular markers. The TriAnnot pipeline is parallelized on a 712 CPU computing cluster that can run a 1-Gb sequence annotation in less than 5 days. It is accessible through a web interface for small scale analyses or through a server for large scale annotations. The performance of TriAnnot was evaluated in terms of sensitivity, specificity, and general fitness using curated reference sequence sets from rice and wheat. In less than 8 h, TriAnnot was able to predict more than 83% of the 3,748 CDS from rice chromosome 1 with a fitness of 67.4%. On a set of 12 reference Mb-sized contigs from wheat chromosome 3B, TriAnnot predicted and annotated 93.3% of the genes among which 54% were perfectly identified in accordance with the reference annotation. It also allowed the curation of 12 genes based on new biological evidences, increasing the percentage of perfect gene prediction to 63%. TriAnnot systematically showed a higher fitness than other annotation pipelines that are not improved for wheat. As it is easily adaptable to the annotation of other plant genomes, TriAnnot should become a useful resource for the annotation of large and complex genomes in the future.

Alternate JournalFront Plant Sci